Issue 21, 2013
From the journal:

Organic & Biomolecular Chemistry

A convenient method for selective detection of 5-hydroxymethylcytosine and 5-formylcytosine sites in DNA sequences

Wuxiang Mao,†a   Jianlin Hu,†a   Tingting Hong,a   Xiwen Xing,a   Sen Wang,a   Xi Chena  and  Xiang Zhou*ab  

* Corresponding authors

a College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan, P. R. of China
E-mail: xzhou@whu.edu.cn
Fax: +96-27-87336380
Tel: +86-27-61056559

b State Key Laboratory of Natural and Biomimetic Drugs, Beijing University, China

Abstract

Treatment of DNA containing 5-formylcytosine with hot piperidine produced a cleaved band at the position of 5-formylcytosine in DNA sequences. After oxidation with KRuO4, 5-hydroxymethylcytosine could also be detected using the same method. Using our strategy, we could detect 5-hydroxymethylcytosine and 5-formylcytosine respectively.

Graphical abstract: A convenient method for selective detection of 5-hydroxymethylcytosine and 5-formylcytosine sites in DNA sequences

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Article information

DOI
https://doi.org/10.1039/C3OB40447A
Article type
Paper
Submitted
05 Mar 2013
Accepted
27 Mar 2013
First published
27 Mar 2013

Org. Biomol. Chem., 2013,11, 3568-3572

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A convenient method for selective detection of 5-hydroxymethylcytosine and 5-formylcytosine sites in DNA sequences

W. Mao, J. Hu, T. Hong, X. Xing, S. Wang, X. Chen and X. Zhou, Org. Biomol. Chem., 2013, 11, 3568 DOI: 10.1039/C3OB40447A

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