Issue 46, 2013
From the journal:

RSC Advances

Chemical tweaking of a non-fluorescent GFP chromophore to a highly fluorescent coumarinic fluorophore: application towards photo-uncaging and stem cell imaging†

Tanmay Chatterjee,a   Debjit Roy,a   Ananya Das,a   Anup Ghosh,a   Partha Pratim Baga  and  Prasun K. Mandal*a  

* Corresponding authors

a Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER)-Kolkata, Mohanpur Campus, West-Bengal, India
E-mail: prasunchem@iiserkol.ac.in

Abstract

Three step chemical tweaking of nonfluorescent parent GFP chromophore has yielded a three hundred times as bright coumarinic fluorophore. A fluorogenic compound has been prepared which after photo-uncaging yields the same highly bright fluorophore that could be used for live stem cell imaging.

Graphical abstract: Chemical tweaking of a non-fluorescent GFP chromophore to a highly fluorescent coumarinic fluorophore: application towards photo-uncaging and stem cell imaging

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Article information

DOI
https://doi.org/10.1039/C3RA44034F
Article type
Communication
Submitted
31 Jul 2013
Accepted
10 Oct 2013
First published
14 Oct 2013
This article is Open Access
Creative Commons BY license

RSC Adv., 2013,3, 24021-24024

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Chemical tweaking of a non-fluorescent GFP chromophore to a highly fluorescent coumarinic fluorophore: application towards photo-uncaging and stem cell imaging

T. Chatterjee, D. Roy, A. Das, A. Ghosh, P. P. Bag and P. K. Mandal, RSC Adv., 2013, 3, 24021 DOI: 10.1039/C3RA44034F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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