Issue 10, 2013
From the journal:

Journal of Materials Chemistry B

A real-time fluorescence turn-on assay for trypsin based on a conjugated polyelectrolyte†

Renxuan Liu,‡ab   Ying Tan,‡b   Cunlong Zhang,b   Jiatao Wu,ab   Lin Mei,b   Yuyang Jiang*bc  and  Chunyan Tan*b  

* Corresponding authors

a The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

b The Ministry-Province Jointly Constructed Base for State Key Lab – Shenzhen Key Laboratory of Chemical Biology, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China
E-mail: tancy@sz.tsinghua.edu.cn
Fax: +86-755-26032094
Tel: +86-755-26036533

c Department of Pharmacology and Pharmaceutical Sciences, School of Medicine, Tsinghua University, Beijing, P. R. China
E-mail: jiangyy@sz.tsinghua.edu.cn
Fax: +86-755-26036017
Tel: +86-755-26036017

Abstract

We report a continuous and sensitive fluorescence turn-on assay for trypsin by using an anionic conjugated polyelectrolyte (PPE-CO2H) and a cationic peptide substrate labelled with p-nitroaniline (RxG-pNA). Applications of the assay in trypsin activity study and high-throughput screening of protease inhibitors were demonstrated.

Graphical abstract: A real-time fluorescence turn-on assay for trypsin based on a conjugated polyelectrolyte

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Article information

DOI
https://doi.org/10.1039/C3TB00020F
Article type
Communication
Submitted
07 Jan 2013
Accepted
23 Jan 2013
First published
24 Jan 2013

J. Mater. Chem. B, 2013,1, 1402-1405

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A real-time fluorescence turn-on assay for trypsin based on a conjugated polyelectrolyte

R. Liu, Y. Tan, C. Zhang, J. Wu, L. Mei, Y. Jiang and C. Tan, J. Mater. Chem. B, 2013, 1, 1402 DOI: 10.1039/C3TB00020F

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