A simple and sensitive UHPLC-MS/MS method for the determination of sulfachloropyrazine sodium in chicken plasma and application in a comparative pharmacokinetic study

Abstract

In this study, a simple and sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for the determination of sulfachloropyrazine sodium (SPZ) using sulfachloropyridazine sodium (SDZ) in chicken plasma has been described. Chromatographic separation was carried out on an octadecylsilyl (ODS) column with gradient elution using 1 mM ammonium formate in water containing 0.1% formic acid and acetonitrile/methanol (v/v, 1 : 1) as the mobile phase at a flow rate of 0.2 mL min⁻¹. Detection was performed by monitoring selective reactions in the negative ion mode. This method was demonstrated to be linear (r > 0.9991) over the concentration range of 0.0025–25 μg mL⁻¹. The intra-day precision ranged from 0.65% to 7.01%, with an accuracy between −8.01% and −0.70%; while the inter-assay precision ranged from 0.42% to 11.32%, with an accuracy between −4.40% and 6.03%. The matrix effects and the extraction recoveries of the analyte and internal standard (IS) from the chicken plasma were within the range of 98.33–103.22% and 92.22–97.13%, respectively. This fully validated method was successfully applied in a pharmacokinetic study in chicken after a single oral administration of SPZ soluble powder (SPZ-SP) and SPZ–diaveridine (SPZ–DVD) suspension at a dose of 30 mg kg⁻¹. Results from this study demonstrate that, after co-administration, DVD can decrease the absorption and prolong the elimination of SPZ.