Issue 14, 2014
From the journal:

Analytical Methods

Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein†

Jinhee Lee,a   Atsuro Tatsumi,a   Koichi Abe,a   Wataru Yoshida,b   Koji Sodea  and  Kazunori Ikebukuro*a  

* Corresponding authors

a Department of Biotechnology and Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan
E-mail: ikebu@cc.tuat.ac.jp
Fax: +81 42 388 7030
Tel: +81 42 388 7030

b School of Biotechnology & Bioscience, Tokyo University of Technology, 1404-1 Katakuramachi, Hachioji City, Tokyo 192-0982, Japan

Abstract

We developed an electrochemical detection system for pathogenic bacteria by utilizing a glucose dehydrogenase-fused zinc finger protein (ZF-GDH), which could detect PCR products electrochemically without the need for DNA probe hybridization. Using ZF-GDH, we could specifically detect 10 copies of genomic DNA derived from Escherichia coli O157.

Graphical abstract: Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

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Article information

DOI
https://doi.org/10.1039/C4AY00977K
Article type
Communication
Submitted
24 Apr 2014
Accepted
22 May 2014
First published
22 May 2014

Anal. Methods, 2014,6, 4991-4994

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Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

J. Lee, A. Tatsumi, K. Abe, W. Yoshida, K. Sode and K. Ikebukuro, Anal. Methods, 2014, 6, 4991 DOI: 10.1039/C4AY00977K

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