Five-plex isotope dimethyl labeling for quantitative proteomics

Yue Wu; Fangjun Wang; Zheyi Liu; Hongqiang Qin; Chunxia Song; Junfeng Huang; Yangyang Bian; Xiaoluan Wei; Jing Dong; Hanfa Zou

doi:10.1039/C3CC47998F

Five-plex isotope dimethyl labeling for quantitative proteomics†

Yue Wu,^a Fangjun Wang,*^a Zheyi Liu,^a Hongqiang Qin,^a Chunxia Song,^a Junfeng Huang,^a Yangyang Bian,^a Xiaoluan Wei,^a Jing Dong^a and Hanfa Zou*^a

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* Corresponding authors

^a Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
E-mail: wangfj@dicp.ac.cn, hanfazou@dicp.ac.cn
Fax: +86-411-84379620
Tel: +86-411-84379610

Abstract

Stable isotope dimethyl labeling, a widely used method for quantitative proteomics, was extended to five channels for the first time. Comprehensive proteome and phosphoproteome quantification validated the high quantification accuracy and throughput of this five-plex method.

Chemical Communications

Five-plex isotope dimethyl labeling for quantitative proteomics†

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Five-plex isotope dimethyl labeling for quantitative proteomics

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