Issue 15, 2014
From the journal:

Chemical Communications

Synthesis of γ-labeled nucleoside 5′-triphosphates using click chemistry†

S. Serdjukow,a   F. Kink,a   B. Steigenberger,a   M. Tomás-Gamasaa  and  T. Carell*a  

* Corresponding authors

a Department of Chemistry, Ludwig-Maximilians Universität München, Butenandtstraße 5-13, 81377 Munich, Germany
E-mail: thomas.carell@cup.uni-muenchen.de

Abstract

Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of γ-alkyne modified triphosphate amidates that are converted into a variety of γ-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.

Graphical abstract: Synthesis of γ-labeled nucleoside 5′-triphosphates using click chemistry

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Article information

DOI
https://doi.org/10.1039/C3CC48937J
Article type
Communication
Submitted
22 Nov 2013
Accepted
19 Dec 2013
First published
20 Dec 2013

Chem. Commun., 2014,50, 1861-1863

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Synthesis of γ-labeled nucleoside 5′-triphosphates using click chemistry

S. Serdjukow, F. Kink, B. Steigenberger, M. Tomás-Gamasa and T. Carell, Chem. Commun., 2014, 50, 1861 DOI: 10.1039/C3CC48937J

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