Issue 63, 2014
From the journal:

Chemical Communications

Clipping of predetermined fragments from the human genome by S1 nuclease–PNA combinations†

Xia Li,a   Satoshi Muneoka,b   Narumi Shigi,ab   Jun Sumaoka‡*ab  and  Makoto Komiyama*ab  

* Corresponding authors

a Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8577 Japan
E-mail: sumaoka@tara.tsukuba.ac.jp, komiyama@tara.tsukuba.ac.jp

b Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904 Japan

Abstract

By combining S1 nuclease with two strands of pseudo-complementary peptide nucleic acid (pcPNA), the whole human genome was selectively cut at targeted sites, and desired fragments were clipped from the genome.

Graphical abstract: Clipping of predetermined fragments from the human genome by S1 nuclease–PNA combinations

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Article information

DOI
https://doi.org/10.1039/C4CC01420K
Article type
Communication
Submitted
24 Feb 2014
Accepted
13 Jun 2014
First published
13 Jun 2014

Chem. Commun., 2014,50, 8674-8676

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Clipping of predetermined fragments from the human genome by S1 nuclease–PNA combinations

X. Li, S. Muneoka, N. Shigi, J. Sumaoka and M. Komiyama, Chem. Commun., 2014, 50, 8674 DOI: 10.1039/C4CC01420K

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