Issue 65, 2014
From the journal:

Chemical Communications

A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications†

K. Engelmark Cassimjee,*a   M. Kadow,a   Y. Wikmark,a   M. Svedendahl Humble,a   M. L. Rothstein,b   D. M. Rothsteinb  and  J.-E. Bäckvall*a  

* Corresponding authors

a Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden
E-mail: karim@organ.su.se, jeb@organ.su.se

b Prime Synthesis, Inc., 2 New Road Suite 126, Aston, PA 19014, USA

Abstract

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.

Graphical abstract: A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

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Article information

DOI
https://doi.org/10.1039/C4CC02605E
Article type
Communication
Submitted
08 Apr 2014
Accepted
20 Jun 2014
First published
03 Jul 2014

Chem. Commun., 2014,50, 9134-9137

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A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

K. Engelmark Cassimjee, M. Kadow, Y. Wikmark, M. Svedendahl Humble, M. L. Rothstein, D. M. Rothstein and J.-E. Bäckvall, Chem. Commun., 2014, 50, 9134 DOI: 10.1039/C4CC02605E

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