Issue 70, 2014
From the journal:

Chemical Communications

Bio-specific and bio-orthogonal chemistries to switch-off the quencher of a FRET-based fluorescent probe: application to living-cell biothiol imaging†

C. Egloff,a   S. A. Jacques,a   M. Nothisen,b   D. Weltin,c   C. Calligaro,c   M. Mosser,a   J.-S. Remyb  and  A. Wagner*a  

* Corresponding authors

a Laboratory of Functional Chemo Systems, UMR 7199, CNRS-UdS, LABEX MEDALIS, UMR 7199 UdS/CNRS, Faculty of Pharmacy, 74 Route du Rhin, 67401 Illkirch-Graffenstaden, France
E-mail: alwag@unistra.fr
Fax: +33-368-854-297

b Laboratory V-SAT, UMR 7199, CNRS-UdS, LABEX MEDALIS, UMR 7199 UdS/CNRS, 74 Route du Rhin, 67401 Illkirch-Graffenstaden, France

c Phytodia SAS, Pôle API, Boulevard Sebastian Brant, 67412 Illkirch-Graffendstaden, France

Abstract

We report the first molecular system that is responsive to both a bio-specific and a bio-orthogonal stimulus. This dual activation process was applied to the design of a biothiol-specific FRET-based fluorescent probe that could be turned-on via an original concept of quencher bleaching.

Graphical abstract: Bio-specific and bio-orthogonal chemistries to switch-off the quencher of a FRET-based fluorescent probe: application to living-cell biothiol imaging

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Article information

DOI
https://doi.org/10.1039/C4CC03548H
Article type
Communication
Submitted
11 May 2014
Accepted
03 Jul 2014
First published
18 Jul 2014

Chem. Commun., 2014,50, 10049-10051

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Bio-specific and bio-orthogonal chemistries to switch-off the quencher of a FRET-based fluorescent probe: application to living-cell biothiol imaging

C. Egloff, S. A. Jacques, M. Nothisen, D. Weltin, C. Calligaro, M. Mosser, J.-S. Remy and A. Wagner, Chem. Commun., 2014, 50, 10049 DOI: 10.1039/C4CC03548H

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