A bioorthogonal 68Ga-labelling strategy for rapid in vivo imaging

Helen L. Evans; Quang-Dé Nguyen; Laurence S. Carroll; Maciej Kaliszczak; Frazer J. Twyman; Alan C. Spivey; Eric O. Aboagye

doi:10.1039/C4CC03903C

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A bioorthogonal ⁶⁸Ga-labelling strategy for rapid in vivo imaging†

Helen L. Evans,^a Quang-Dé Nguyen,^a Laurence S. Carroll,^a Maciej Kaliszczak,^a Frazer J. Twyman,^a Alan C. Spivey^b and Eric O. Aboagye*^a

Author affiliations

* Corresponding authors

^a Comprehensive Cancer Imaging Centre, Department of Surgery & Cancer, Imperial College London, Hammersmith Hospital, London W12 0NN, UK
E-mail: e.aboagye@imperial.ac.uk

^b Department of Chemistry, Imperial College London, South Kensington Campus, London SW7 2AZ, UK

Abstract

Herein, we describe a fast and robust method for achieving ⁶⁸Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels–Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.

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Article information

DOI: https://doi.org/10.1039/C4CC03903C
Article type: Communication
Submitted: 21 May 2014
Accepted: 04 Jul 2014
First published: 04 Jul 2014

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Chem. Commun., 2014,50, 9557-9560

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A bioorthogonal ⁶⁸Ga-labelling strategy for rapid in vivo imaging

H. L. Evans, Q. Nguyen, L. S. Carroll, M. Kaliszczak, F. J. Twyman, A. C. Spivey and E. O. Aboagye, Chem. Commun., 2014, 50, 9557 DOI: 10.1039/C4CC03903C

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