Issue 78, 2014
From the journal:

Chemical Communications

Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding†

Chen Sun,a   Mingxing Ouyang,b   Zhenning Cao,a   Sai Ma,a   Hamzeh Alqublan,c   Nammalwar Sriranganathan,c   Yingxiao Wangb  and  Chang Lu*d  

* Corresponding authors

a School of Biomedical Engineering and Sciences, Virginia Tech-Wake Forest University, Blacksburg, Virginia, USA

b Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, California, USA

c Department of Biomedical Sciences & Pathobiology, Virginia Tech, Blacksburg, Virginia, USA

d Department of Chemical Engineering, Virginia Tech, Blacksburg, Virginia, USA
E-mail: changlu@vt.edu
Fax: +1 540 231 5022
Tel: +1 540 231 8681

Abstract

Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ∼100 cells with high spatiotemporal resolution.

Graphical abstract: Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

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Article information

DOI
https://doi.org/10.1039/C4CC04730C
Article type
Communication
Submitted
21 Jun 2014
Accepted
07 Aug 2014
First published
08 Aug 2014
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2014,50, 11536-11539

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Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

C. Sun, M. Ouyang, Z. Cao, S. Ma, H. Alqublan, N. Sriranganathan, Y. Wang and C. Lu, Chem. Commun., 2014, 50, 11536 DOI: 10.1039/C4CC04730C

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