The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
G. R. McInroy, E. Raiber and S. Balasubramanian, Chem. Commun., 2014, 50, 12047 DOI: 10.1039/C4CC05107F
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