Issue 80, 2014
From the journal:

Chemical Communications

A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases†

Guojie Zhao,a   Tianyu Hu,a   Jun Li,a   Hua Wei,b   Hong Shangc  and  Yifu Guan*a  

* Corresponding authors

a Key Laboratory of Medical Cell Biology (Ministry of Education), Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning, China
E-mail: yfguan@mail.cmu.edu.cn

b Animal Science and Veterinary Medicine College, Shenyang Agricultural University, Shenyang, Liaoning, China

c Department of Clinical Diagnosis, the First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China

Abstract

We have developed a rapid and high-throughput assay based on rolling circle amplification, to distinguish individual strand cleavage of DNA duplexes by restriction endonucleases. As an illustration, we analyzed nicking activity of Nb.BbvCI and uneven cleavage of LNA modified DNA by EcoRI. This assay has potential for analyzing protein–DNA interactions.

Graphical abstract: A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases

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Article information

DOI
https://doi.org/10.1039/C4CC05314A
Article type
Communication
Submitted
12 Jul 2014
Accepted
13 Aug 2014
First published
13 Aug 2014

Chem. Commun., 2014,50, 11930-11933

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A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases

G. Zhao, T. Hu, J. Li, H. Wei, H. Shang and Y. Guan, Chem. Commun., 2014, 50, 11930 DOI: 10.1039/C4CC05314A

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