Issue 91, 2014
From the journal:

Chemical Communications

The phosphatase mechanism of bifunctional kinase/phosphatase AceK†

Shu Wang,a   Qingya Shen,a   Guangju Chen,a   Jimin Zheng,*a   Hongwei Tan*a  and  Zongchao Jiaab  

* Corresponding authors

a College of Chemistry, Beijing Normal University, Beijing, China
E-mail: jimin_z@bnu.edu.cn, hongwei.tan@bnu.edu.cn

b Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada K7L 3N6

Abstract

We have revealed that bifunctional AceK kinase/phosphatase utilizes a stepwise addition–elimination mechanism in its dephosphorylation reaction. This work explains how AceK enables opposite kinase and phosphatase activities with Asp477 and a single Mg2+ ion.

Graphical abstract: The phosphatase mechanism of bifunctional kinase/phosphatase AceK

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Article information

DOI
https://doi.org/10.1039/C4CC05375C
Article type
Communication
Submitted
18 Jul 2014
Accepted
17 Sep 2014
First published
17 Sep 2014

Chem. Commun., 2014,50, 14117-14120

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The phosphatase mechanism of bifunctional kinase/phosphatase AceK

S. Wang, Q. Shen, G. Chen, J. Zheng, H. Tan and Z. Jia, Chem. Commun., 2014, 50, 14117 DOI: 10.1039/C4CC05375C

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