Detection of multiple mutations in a single codon of genomic DNA

Abstract

The controller DNA technology allows the detection of multiple mutations in a single codon of genomic DNA. In this technology, the controller DNA is used to control the hybridization of target DNAs with the immobilized DNAs. The controller DNA technology is rapid, specific, and cost-effective for the following reasons, (i) final results in 40 min after PCR, (ii) detection and discrimination of the six mutations in a single codon, and (iii) high sensitivity.