Issue 91, 2014
From the journal:

Chemical Communications

Enhanced DNA toehold exchange reaction on a chip surface to discriminate single-base changes†

Huaguo Xu,a   Wei Deng,a   Fujian Huang,*a   Shiyan Xiao,a   Gang Liub  and  Haojun Liang*ac  

* Corresponding authors

a CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China
E-mail: fjhuang@ustc.edu.cn, hjliang@ustc.edu.cn

b National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China

c Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China

Abstract

In this study, a single-base change discrimination strategy was developed through enhanced DNA toehold exchange reaction on a chip surface. A DNA strand with single-base change (SNPs, insertion or deletion) at an arbitrary position was clearly discriminated with nucleic acid concentrations from 0.8 nM to 1 μM through this method.

Graphical abstract: Enhanced DNA toehold exchange reaction on a chip surface to discriminate single-base changes

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4CC07272C
Article type
Communication
Submitted
15 Sep 2014
Accepted
24 Sep 2014
First published
25 Sep 2014

Chem. Commun., 2014,50, 14171-14174

Permissions

Request permissions

Enhanced DNA toehold exchange reaction on a chip surface to discriminate single-base changes

H. Xu, W. Deng, F. Huang, S. Xiao, G. Liu and H. Liang, Chem. Commun., 2014, 50, 14171 DOI: 10.1039/C4CC07272C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements