Issue 4, 2014

Adsorption of NGF and BDNF derived peptides on gold surfaces

Abstract

This study tackles the interaction between gold surfaces and two peptide fragments named NGF(1-14) and BDNF(1-12), able to mimic the proliferative activity of the nerve growth factor (NGF) and the brain derived neurotrophic factor (BDNF), respectively. The physical adsorption processes on the solid surface from both single and binary peptide solutions, at physiological and acid pH, were investigated by QCM-D and CD experiments, as well as by molecular dynamics calculations. The relevant physicochemical properties at the hybrid bio-interface, including peptide–surface interaction, conformational changes, hydrodynamic thickness, viscoelastic parameters, and competitive vs. synergic behaviour of the two peptide fragments towards the surface, were scrutinized. Biological assays with neuronal cells pointed to the maintenance of the biological activity of NGF(1-14) and BDNF(1-12) peptide molecules within the adlayers on the gold surface.

Graphical abstract: Adsorption of NGF and BDNF derived peptides on gold surfaces

Supplementary files

Article information

Article type
Paper
Submitted
16 Jun 2013
Accepted
14 Nov 2013
First published
14 Nov 2013

Phys. Chem. Chem. Phys., 2014,16, 1536-1544

Adsorption of NGF and BDNF derived peptides on gold surfaces

G. Forte, A. Travaglia, A. Magrì, C. Satriano and D. La Mendola, Phys. Chem. Chem. Phys., 2014, 16, 1536 DOI: 10.1039/C3CP52499J

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