Issue 30, 2014

A salt-bridge structure in solution revealed by 2D-IR spectroscopy

Abstract

Salt bridges are important interactions for the stability of protein conformations, but up to now it has been difficult to determine salt-bridge geometries in solution. Here we characterize the spatial structure of a salt bridge between guanidinium (Gdm+) and acetate (Ac) using two-dimensional vibrational (2D-IR) spectroscopy. We find that as a result of salt bridge formation there is a significant change in the infrared response of Gdm+ and Ac, and cross peaks between them appear in the 2D-IR spectrum. From the 2D-IR spectrum we determine the relative orientation of the transition-dipole moments of the vibrational modes of Gdm+ and Ac, as well as the coupling between them.

Graphical abstract: A salt-bridge structure in solution revealed by 2D-IR spectroscopy

Supplementary files

Article information

Article type
Communication
Submitted
16 Jan 2014
Accepted
11 Mar 2014
First published
17 Mar 2014
This article is Open Access
Creative Commons BY license

Phys. Chem. Chem. Phys., 2014,16, 15784-15786

Author version available

A salt-bridge structure in solution revealed by 2D-IR spectroscopy

A. Huerta-Viga, S. R. Domingos, S. Amirjalayer and S. Woutersen, Phys. Chem. Chem. Phys., 2014, 16, 15784 DOI: 10.1039/C4CP00233D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements