Issue 18, 2014
From the journal:

Nanoscale

Transient absorption microscopy of gold nanorods as spectrally orthogonal labels in live cells†

Tao Chen,‡a   Shouhui Chen,‡b   Jihan Zhou,c   Dehai Liang,c   Xiaoyuan Chen*d  and  Yanyi Huang*a  

* Corresponding authors

a Biodynamic Optical Imaging Center (BIOPIC), College of Engineering, Peking University, Beijing 100871, China
E-mail: yanyi@pku.edu.cn

b Med-X Research Institutes, Shanghai Jiaotong University, Shanghai 200030, China

c Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

d Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA
E-mail: shawn.chen@nih.gov

Abstract

Gold nanorods (AuNRs) have shown great potential as bio-compatible imaging probes in various biological applications. Probing nanomaterials in live cells is essential to reveal the interaction between them. In this study, we used a transient absorption microscope to selectively image AuNRs in live cells. The transient absorption signals were monitored through lock-in amplification. This provides a new way of observing AuNRs with no interference from background autofluorescence.

Graphical abstract: Transient absorption microscopy of gold nanorods as spectrally orthogonal labels in live cells

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Article information

DOI
https://doi.org/10.1039/C4NR03413A
Article type
Communication
Submitted
19 Jun 2014
Accepted
10 Jul 2014
First published
11 Jul 2014

Nanoscale, 2014,6, 10536-10539

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Transient absorption microscopy of gold nanorods as spectrally orthogonal labels in live cells

T. Chen, S. Chen, J. Zhou, D. Liang, X. Chen and Y. Huang, Nanoscale, 2014, 6, 10536 DOI: 10.1039/C4NR03413A

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