Issue 8, 2014
From the journal:

Organic & Biomolecular Chemistry

Synthesis, biophysical studies and RNA interference activity of RNA having three consecutive amide linkages†

Paul Tanui,a   Scott D. Kennedy,b   Benjamin D. Lunstad,c   Amanda Haas,c   Devin Leakec  and  Eriks Rozners*a  

* Corresponding authors

a Department of Chemistry, Binghamton University, The State University of New York, Binghamton, New York 13902, USA
E-mail: erozners@binghamton.edu

b Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA

c Global Research and Development in Molecular Biology, Thermo Fisher Scientific Bioscience Division, Lafayette, Colorado 80026, USA

Abstract

RNA sequences having up to three consecutive internal amide linkages were synthesized and studied using UV and NMR spectroscopy. The amide modifications did not interfere with normal base-pairing and A-type RNA conformation. Three consecutive amides were well tolerated in the passenger strand of siRNA and caused little change in RNAi activity.

Graphical abstract: Synthesis, biophysical studies and RNA interference activity of RNA having three consecutive amide linkages

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Article information

DOI
https://doi.org/10.1039/C3OB42532K
Article type
Communication
Submitted
18 Dec 2013
Accepted
06 Jan 2014
First published
07 Jan 2014

Org. Biomol. Chem., 2014,12, 1207-1210

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Synthesis, biophysical studies and RNA interference activity of RNA having three consecutive amide linkages

P. Tanui, S. D. Kennedy, B. D. Lunstad, A. Haas, D. Leake and E. Rozners, Org. Biomol. Chem., 2014, 12, 1207 DOI: 10.1039/C3OB42532K

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