Issue 26, 2014

RNA–peptide conjugate synthesis by inverse-electron demand Diels–Alder reaction

Abstract

Here we report an efficient method for the synthesis of RNA–peptide conjugates by inverse-electron demand Diels–Alder reaction. Various dienophiles were enzymatically incorporated into RNA and reacted with a chemically synthesized diene-modified peptide. The Diels–Alder reaction proceeds with near-quantitative yields in aqueous solution with stoichiometric amounts of reactants, even at low micromolar concentrations.

Graphical abstract: RNA–peptide conjugate synthesis by inverse-electron demand Diels–Alder reaction

Supplementary files

Article information

Article type
Paper
Submitted
09 Jan 2014
Accepted
20 May 2014
First published
20 May 2014

Org. Biomol. Chem., 2014,12, 4701-4707

Author version available

RNA–peptide conjugate synthesis by inverse-electron demand Diels–Alder reaction

S. Ameta, J. Becker and A. Jäschke, Org. Biomol. Chem., 2014, 12, 4701 DOI: 10.1039/C4OB00076E

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