We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.
D. Li, X. Wang, F. Shi, R. Sha, N. C. Seeman and J. W. Canary, Org. Biomol. Chem., 2014, 12, 8823 DOI: 10.1039/C4OB01552E
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