Characterization of the antioxidant activity of gold@platinum nanoparticles

Abstract

Gold@platinum (Au@Pt) nanostructures exhibit desirable catalytic abilities and unique optical properties, which are not seen in single-component metal nanoparticles (NPs). To date, however, there is only limited information available in the literature on the integrated approach to investigate the antioxidant activities of Au@Pt NPs. The aim of the present study, was to evaluate the antioxidant activities of hybrid nanorods, i.e., Au@Pt NPs. Specifically, the free radical scavenging effect, hydroxyl radical scavenging activity, and superoxide dismutase (SOD)-like activity of Au@Pt NPs were examined. Furthermore, the in vitro antioxidant ability of Au@Pt NPs was assessed by measuring the cell viability of V79-4 (Chinese hamster lung fibroblasts), PC-3 (human prostate cancer), and MCF-7 (human breast cancer) cells under oxidative stress induced by hydrogen peroxide (H₂O₂). The results showed that Au@Pt NPs exhibited high DPPH radical scavenging activity, non-site specific hydroxyl radical scavenging activity, linoleic acid peroxidation inhibitory ability by TBA tests in a low Au@Pt NP concentration range. Furthermore, in vitro experiments revealed that Au@Pt NPs showed cellular protection against oxidative stress induced by H₂O₂.