In this study, we observed that 5′-end 6-mercapto-1-hexane-labeled single-stranded DNA (HS-ssDNA) dramatically enhanced the specificity of DNA duplication during the polymerase chain reaction (PCR). The regulation of PCR by HS-ssDNA is both intriguing and useful, and such findings further our understanding of the biochemical characteristics and potential applications of HS-ssDNA.
G. Zhang, H. Sun, F. Li, S. Yang, R. Gao, X. Xu and X. Lu, RSC Adv., 2014, 4, 34591 DOI: 10.1039/C4RA04671D
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