A paper based microfluidic device for easy detection of uric acid using positively charged gold nanoparticles

Abstract

A paper based microfluidic device is fabricated that can rapidly detect very low concentrations of uric acid (UA) using 3,5,3′,5′-tetramethyl benzidine (TMB), H₂O₂ and positively charged gold nanoparticles ((+)AuNPs). In the presence of (+)AuNPs, H₂O₂ reacts with TMB to produce a bluish-green colour which becomes colourless on reaction with UA. This colorimetric method can detect as low as 8.1 ppm of UA within <20 minutes on white filter paper. This technique provides an alternative way for UA detection.