A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.
L. Hakuna, B. Doughan, J. O. Escobedo and R. M. Strongin, Analyst, 2015, 140, 3339 DOI: 10.1039/C5AN00345H
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