Issue 13, 2015
From the journal:

Analyst

A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature

Seiichi Uchiyama,*a   Toshikazu Tsuji,*ab   Kumiko Ikado,b   Aruto Yoshida,b   Kyoko Kawamoto,a   Teruyuki Hayashic  and  Noriko Inadac  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: seiichi@mol.f.u-tokyo.ac.jp

b Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan
E-mail: Toshikazu_2_Tsuji@kirin.co.jp

c Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan

Abstract

We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01–1.0 °C) using the new cationic fluorescent polymeric thermometer.

Graphical abstract: A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature

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Article information

DOI
https://doi.org/10.1039/C5AN00420A
Article type
Paper
Submitted
04 Mar 2015
Accepted
01 May 2015
First published
01 May 2015
This article is Open Access
Creative Commons BY-NC license

Analyst, 2015,140, 4498-4506

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A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature

S. Uchiyama, T. Tsuji, K. Ikado, A. Yoshida, K. Kawamoto, T. Hayashi and N. Inada, Analyst, 2015, 140, 4498 DOI: 10.1039/C5AN00420A

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