Issue 17, 2015
From the journal:

Analyst

Hyperpolarized NMR of plant and cancer cell extracts at natural abundance†

Jean-Nicolas Dumez,a   Jonas Milani,b   Basile Vuichoud,b   Aurélien Bornet,b   Julie Lalande-Martin,c   Illa Tea,cd   Maxime Yon,ce   Mickaël Maucourt,fg   Catherine Deborde,fh   Annick Moing,fh   Lucio Frydman,e   Geoffrey Bodenhausen,bijk   Sami Jannin*b  and  Patrick Giraudeau*cl  

* Corresponding authors

a Institut de Chimie des Substances Naturelles, CNRS UPR 2301, F-91190 Gif-sur-Yvette, France

b Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland
E-mail: sami.jannin@epfl.ch

c Université de Nantes, CNRS, CEISAM UMR 6230, F-44322 Nantes Cedex 03, France

d Cancer Metabolism and Genetics Group, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT2601, Australia

e Department of Chemical Physics, Weizmann Institute of Science, IL-76100 Rehovot, Israel

f Plateforme Métabolome Bordeaux – MetaboHUB, Centre de Génomique Fonctionnelle Bordeaux, IBVM, Centre INRA Bordeaux, F-33140 Villenave d'Ornon, France

g Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, Centre INRA Bordeaux, F-33140 Villenave d'Ornon, France

h INRA, UMR 1332 Biologie du Fruit et Pathologie, Centre INRA Bordeaux, F-33140 Villenave d'Ornon, France

i Département de Chimie, Ecole Normale Supérieure (ENS)-Paris Sciences Lettres (PSL) Research University, F-75005 Paris, France

j Laboratoire de Biomolécules (LBM), Université Pierre et Marie Curie (UPMC) – Paris 06, Sorbonne Universités, F-75005 Paris, France

k Laboratoire de Biomolécules (LBM), Unité Mixte de Recherche (UMR) 7203 Centre National de la Recherche Scientifique (CNRS), F-75005 Paris, France

l Institut Universitaire de France, F-75005 Paris, France
E-mail: patrick.giraudeau@univ-nantes.fr

Abstract

Natural abundance 13C NMR spectra of biological extracts are recorded in a single scan provided that the samples are hyperpolarized by dissolution dynamic nuclear polarization combined with cross polarization. Heteronuclear 2D correlation spectra of hyperpolarized breast cancer cell extracts can also be obtained in a single scan. Hyperpolarized NMR of extracts opens many perspectives for metabolomics.

Graphical abstract: Hyperpolarized NMR of plant and cancer cell extracts at natural abundance

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Article information

DOI
https://doi.org/10.1039/C5AN01203A
Article type
Communication
Submitted
15 Jun 2015
Accepted
20 Jul 2015
First published
20 Jul 2015

Analyst, 2015,140, 5860-5863

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Hyperpolarized NMR of plant and cancer cell extracts at natural abundance

J. Dumez, J. Milani, B. Vuichoud, A. Bornet, J. Lalande-Martin, I. Tea, M. Yon, M. Maucourt, C. Deborde, A. Moing, L. Frydman, G. Bodenhausen, S. Jannin and P. Giraudeau, Analyst, 2015, 140, 5860 DOI: 10.1039/C5AN01203A

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