Issue 21, 2015

Electrogenerated chemiluminescence of tris(2,2′ bipyridine)ruthenium(ii) using common biological buffers as co-reactant, pH buffer and supporting electrolyte

Abstract

A series of aliphatic tertiary amines (HEPES, POPSO, EPPS and BIS-TRIS) commonly used to buffer the pH in biological experiments, were examined as alternative, non-toxic co-reactants for the electrogenerated chemiluminescence (ECL) of tris(2,2′-bipyridine)ruthenium(II) ([Ru(bpy)3]2+). These were found to be very attractive as “multi-tasking” reagents, serving not only as co-reactants, but also fulfiling the roles of pH buffer and supporting electrolyte within an aqueous environment; thus significantly simplifying the overall ECL analysis. Sub-nanomolar detection limits were obtained for [Ru(bpy)3]2+ in the presence of BIS-TRIS, making this species an valuable option for co-reactant ECL-based bioanalytical applications.

Graphical abstract: Electrogenerated chemiluminescence of tris(2,2′ bipyridine)ruthenium(ii) using common biological buffers as co-reactant, pH buffer and supporting electrolyte

Supplementary files

Article information

Article type
Communication
Submitted
17 Jun 2015
Accepted
28 Aug 2015
First published
28 Aug 2015
This article is Open Access
Creative Commons BY-NC license

Analyst, 2015,140, 7142-7145

Author version available

Electrogenerated chemiluminescence of tris(2,2′ bipyridine)ruthenium(II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte

N. Kebede, P. S. Francis, G. J. Barbante and C. F. Hogan, Analyst, 2015, 140, 7142 DOI: 10.1039/C5AN01216C

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