Enhanced sensing performance of supported graphitic carbon nitride nanosheets and the fabrication of electrochemiluminescent biosensors for IgG

Abstract

The key challenge for fabricating electrogenerated chemiluminescence (ECL) biosensors still lies in the improvement of the sensing performance of ECL luminophores. Graphite-like carbon nitride (g-C₃N₄) nanosheets were prepared and the cathodic ECL performance was investigated. The ECL emission of g-C₃N₄ nanosheets was greatly enhanced and higher sensing sensitivity towards copper ions was achieved by a “space effect”, the incorporation of g-C₃N₄ nanosheets into a top-down prepared nanoporous matrix. Label-free ECL immunosensors were fabricated based on this simple sensing platform. The unique signal amplification strategy and simple fabrication procedure guarantee the sensor stability and production reproducibility. Nanoporous matrix supported ECL probes have potential as a versatile sensing strategy for the fabrication of ECL biosensors.