Issue 2, 2015

Analysis of steroid hormones in human saliva by matrix-assisted laser desorption/ionization mass spectrometry

Abstract

This work describes a simple and rapid method for the quantitative analysis of four steroid hormones, including testosterone, progesterone, hydrocortisone and cortisone in human saliva samples by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry using nanomaterials as the matrix. The optimal pretreatment processes of saliva samples as well as MALDI conditions for efficient desorption/ionization of these steroid hormones are systematically explored. Parameters, including the concentration and pH level of ammonium citrate buffer, the nature and concentration of the MALDI matrix, laser energy, and the sample extraction method, were systematically investigated in this study. The limits of detection at a signal to noise ratio of 3 for testosterone, progesterone, hydrocortisone and cortisone provided by the proposed approach are 6.0, 6.0, 6.0, and 3.0 μM, respectively, with satisfactory quantitative linearity (R2 > 0.985) and recovery (89.3–111.2%) for the four analytes. Our technique provides the advantages of sensitivity, repeatability, speed, and simplicity for the quantitation of the four steroid hormones in saliva samples. This method holds great potential for high-throughput biological screening and disease detection.

Graphical abstract: Analysis of steroid hormones in human saliva by matrix-assisted laser desorption/ionization mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
12 Aug 2014
Accepted
08 Nov 2014
First published
10 Nov 2014

Anal. Methods, 2015,7, 486-489

Author version available

Analysis of steroid hormones in human saliva by matrix-assisted laser desorption/ionization mass spectrometry

C. Chen, M. Bair, C. Hsu, T. Chiu and C. Hu, Anal. Methods, 2015, 7, 486 DOI: 10.1039/C4AY01897D

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