Issue 1, 2015

Detection of Fe(iii) and bio-copper in human serum based on fluorescent AuAg nanoclusters

Abstract

In this study, a fluorescence assay for the successive determination of Fe3+ and Cu2+ ions based on the quenching fluorescence of composite AuAg nanoclusters (AuAg NCs) was developed. Using this binary fluorescence sensor, the Fe(III) level in a human serum sample can be directly detected without pretreatment. After the nitrification of human serum, the bio-copper level in human serum may be measured with a quick response. Human serum samples were analyzed, and the average concentration of Fe(III) and bio-copper were found to be 2.33 × 10−5 and 2.91 × 10−5 M, respectively. This assay was not only sensitively responsive to blood iron(III) but also to serum copper, suggesting significant potential applications for successively monitoring the Fe(III) and bio-copper levels, and their changes during the progression of a biological process.

Graphical abstract: Detection of Fe(iii) and bio-copper in human serum based on fluorescent AuAg nanoclusters

Supplementary files

Article information

Article type
Paper
Submitted
28 Sep 2014
Accepted
04 Nov 2014
First published
04 Nov 2014

Anal. Methods, 2015,7, 296-300

Author version available

Detection of Fe(III) and bio-copper in human serum based on fluorescent AuAg nanoclusters

Q. Zhao, S. Chen, L. Zhang and H. Huang, Anal. Methods, 2015, 7, 296 DOI: 10.1039/C4AY02297A

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