Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions

Xiaoxia Lu; Nandi Zhou; Yaping Tian

doi:10.1039/C4AY02693D

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Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions†

Xiaoxia Lu,^a Nandi Zhou^a and Yaping Tian*^a

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* Corresponding authors

^a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
E-mail: biochem@jiangnan.edu.cn
Fax: +86 510 85918116
Tel: +86 510 85918116

Abstract

A highly sensitive spectrophotometric method for ethyl carbamate (EC) determination was established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range of 0.3–50 μM, with a low detection limit of 0.00928 μM. The assay was further applied to analyse EC in mimic Chinese rice wine samples.

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Article information

DOI: https://doi.org/10.1039/C4AY02693D
Article type: Communication
Submitted: 12 Nov 2014
Accepted: 04 Dec 2014
First published: 05 Dec 2014

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Anal. Methods, 2015,7, 1261-1264

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Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions

X. Lu, N. Zhou and Y. Tian, Anal. Methods, 2015, 7, 1261 DOI: 10.1039/C4AY02693D

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Analytical Methods

Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions†

Abstract

Supplementary files

Article information

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Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions

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