A novel label-free biosensor based on self-assembled aptamer/GO architecture for sensitive detection of biomolecules

Abstract

We developed a novel label-free biosensor for biomolecule detection based on the thioflavin T (ThT)-induced conformational change of guanine-rich oligonucleotides and self-assembled aptamer/GO nanosheet architecture. In the presence of target biomolecules, the aptamer sequence could specifically bind to the target and release from the surface of GO nanosheets to form a G-quadruplex conformation with ThT as an inducer, resulting in enhancement of fluorescence. The proposed biosensor exhibits a “turn-on” signal, which allows sensitive, selective and rapid detection of biomolecules.