A label-free fluorescent sensor for the detection of Pb2+ and Hg2+

Hanchu Xu; Shenshan Zhan; Dongwei Zhang; Bing Xia; Xuejia Zhan; Lumei Wang; Pei Zhou

doi:10.1039/C5AY01282A

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A label-free fluorescent sensor for the detection of Pb²⁺ and Hg²⁺†

Hanchu Xu,^a Shenshan Zhan,^a Dongwei Zhang,^b Bing Xia,^b Xuejia Zhan,^a Lumei Wang^a and Pei Zhou*^a

Author affiliations

* Corresponding authors

^a School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, China
E-mail: zhoupei@sjtu.edu.cn
Fax: +86-21-34205762
Tel: +86-21-34205762

^b School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, China

Abstract

A label-free fluorescent DNA-based sensor for the detection of Pb²⁺ and Hg²⁺ has been reported in this paper. A single-stranded DNA named modified T30695 was used as a recognition probe and SYBR Green I (SG) was used as a signal reporter. This sensor consisted of two interaction sections: Pb²⁺ interacts with modified T30695 to form a G-quadruplex and Hg²⁺ interacts with modified T30695 to form a T–Hg(II)–T hairpin conformation. Circular dichroism confirmed the interactions between modified T30695 and Pb²⁺ or Hg²⁺. Based on this, a sensor for the detection of Pb²⁺ and Hg²⁺ with limits of detection of 2.09 ppb and 1.14 ppb was constructed.

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Article information

DOI: https://doi.org/10.1039/C5AY01282A
Article type: Paper
Submitted: 18 May 2015
Accepted: 24 Jun 2015
First published: 24 Jun 2015

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Anal. Methods, 2015,7, 6260-6265

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A label-free fluorescent sensor for the detection of Pb²⁺ and Hg²⁺

H. Xu, S. Zhan, D. Zhang, B. Xia, X. Zhan, L. Wang and P. Zhou, Anal. Methods, 2015, 7, 6260 DOI: 10.1039/C5AY01282A

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Analytical Methods