Issue 21, 2015

An electrochemical immunosensor for ultrasensitive detection of HBsAg based on platinum nanoparticles loaded on natural montmorillonite

Abstract

A sensitive and facile electrochemical immunosensor for ultrasensitive detection of hepatitis B surface antigen (HBsAg) was designed, which was based on platinum nanoparticle decorated amino silane functionalized montmorillonite (Pt–NH2–MMT). As it is a luminosilicate clay mineral, the advantages of montmorillonite (MMT), such as a large specific surface area, good adsorption/ion exchange capacity and innocuousness, endow Pt–NH2–MMT with better sensing performance. The platinum nanoparticles (Pt NPs) also play an important role due to their good biological compatibility, electron transport rate and catalytic activity. Pt NPs and the available amine group of second HBsAg-antibody could be bound strongly to each other. And the covalent bond between NH2–MMT and the carboxyl group of primary HBsAg-antibody could be formed by the activation of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide. Under optimal conditions, the Pt–NH2–MMT showed high electrocatalytic activity toward hydrogen peroxide reduction. Under optimal conditions, the immunosensor showed a wide linear response to HBsAg ranging from 0.5 pg mL−1 to 20 ng mL−1 with a low detection limit of 2.0 × 10−4 ng mL−1. The designed immunosensor displayed high sensitivity, stability and reproducibility for the analysis of HBsAg.

Graphical abstract: An electrochemical immunosensor for ultrasensitive detection of HBsAg based on platinum nanoparticles loaded on natural montmorillonite

Supplementary files

Article information

Article type
Paper
Submitted
18 Jun 2015
Accepted
11 Sep 2015
First published
15 Sep 2015

Anal. Methods, 2015,7, 9150-9157

Author version available

An electrochemical immunosensor for ultrasensitive detection of HBsAg based on platinum nanoparticles loaded on natural montmorillonite

Y. Li, L. Tian, H. Jia, X. Pang, W. Cao and Q. Wei, Anal. Methods, 2015, 7, 9150 DOI: 10.1039/C5AY01574J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements