Issue 19, 2015

Bovine serum albumin-capped CdS quantum dots as an inner-filter effect sensor for rapid detection and quantification of protamine and heparin

Abstract

In this work, we developed a novel and sensitive sensor for the detection of heparin and protamine based on the inner-filter effect (IFE) between gold nanoparticles (AuNPs) and bovine serum albumin-capped CdS quantum dots (QDs). The methodology enabled the use of a simple synthesis procedure for CdS QDs via a fast route using bovine serum albumin as a capping agent. The fluorescence of the QDs could be quenched by AuNPs based on the IFE. Protamine could effectively turn on the fluorescence due to the electrostatic attraction between protamine and the AuNPs. On the addition of both heparin and protamine, the IFE system was formed owing to the stronger interaction between heparin and protamine than that with the AuNPs, leading to a marked fluorescence quenching of the QDs. By measuring the fluorescence of the QDs, the concentration of protamine and heparin was evaluated. Under the optimized conditions, the proposed nanosensor could be used for protamine detection in the concentration range of 10–350 ng mL−1 with a detection limit of 2.4 ng mL−1. The linear range for heparin anions was 10–300 ng mL−1 with a detection limit of 2.2 ng mL−1. Furthermore, a simple and convenient method had been used for protamine and heparin detection in serum samples with satisfactory results.

Graphical abstract: Bovine serum albumin-capped CdS quantum dots as an inner-filter effect sensor for rapid detection and quantification of protamine and heparin

Supplementary files

Article information

Article type
Paper
Submitted
13 Jul 2015
Accepted
24 Aug 2015
First published
25 Aug 2015

Anal. Methods, 2015,7, 8445-8452

Bovine serum albumin-capped CdS quantum dots as an inner-filter effect sensor for rapid detection and quantification of protamine and heparin

H. Li and X. Yang, Anal. Methods, 2015, 7, 8445 DOI: 10.1039/C5AY01817J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements