Issue 5, 2015
From the journal:

Chemical Communications

A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity†

Jing Tao,a   Panshu Song,b   Yusuke Sato,c   Seiichi Nishizawa,c   Norio Teramae,c   Aijun Tonga  and  Yu Xiang*a  

* Corresponding authors

a Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China
E-mail: xiang-yu@tsinghua.edu.cn

b National Institute of Metrology, Beijing 100029, China

c Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan

Abstract

The activity of uracil-DNA glycosylase (UDG), an enzyme in the base excision repair, is detected at a high sensitivity by a DNA substrate containing only one uracil through a label-free fluorescent approach, which is also successfully applied for the measurement of UDG inhibitors.

Graphical abstract: A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

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Article information

DOI
https://doi.org/10.1039/C4CC06170E
Article type
Communication
Submitted
07 Aug 2014
Accepted
04 Nov 2014
First published
04 Nov 2014

Chem. Commun., 2015,51, 929-932

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A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

J. Tao, P. Song, Y. Sato, S. Nishizawa, N. Teramae, A. Tong and Y. Xiang, Chem. Commun., 2015, 51, 929 DOI: 10.1039/C4CC06170E

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