Issue 1, 2015
From the journal:

Chemical Communications

Reversible biomechano-responsive surface based on green fluorescent protein genetically modified with unnatural amino acids†

Johan Longo,‡a   Chunyan Yao,‡b   César Rios,a   Nguyet Trang Thanh Chau,a   Fouzia Boulmedais,ac   Joseph Hemmerlé,d   Philippe Lavalle,d   Stefan M. Schiller,*befgh   Pierre Schaaf*ad  and  Loïc Jierryac  

* Corresponding authors

a ICS (UPR22-CNRS), 23 rue du Loess, Strasbourg, France
E-mail: schaaf@unistra.fr

b Universität Freiburg, Albertstrasse 19, Freiburg im Breisgau, Germany
E-mail: Stefan.Schiller@ZBSA.Uni-Freiburg.de

c USIAS, 5 allée du Général Rouvillois, 67083 Strasbourg, France

d INSERM, UMR-S 1121, 11 rue Humann, 67085 Strasbourg Cedex, France

e Institute for Pharmaceutical Sciences, Albertstr. 25, Germany

f FRIAS, School of Soft Matter Research, Albertstr. 19, Germany

g BIOSS Centre for Biological Signalling Studies, Univ. of Freiburg, Schänzlestrasse 18, D-79104 Freiburg, Germany

h Dep. of Microsystems Engineering, Univ. of Freiburg, Georges-Köhler-Allee 102, D-79110 Freiburg, Germany

Abstract

GFP has been genetically modified at two specific positions of its molecular architecture. These modifications allow its covalent attachment onto PEG brushes grafted on functionalized silicone surfaces. The stretching of this material leads to a reversible decrease of the fluorescence intensity due to stretch-induced forces applying on GFP molecules.

Graphical abstract: Reversible biomechano-responsive surface based on green fluorescent protein genetically modified with unnatural amino acids

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Article information

DOI
https://doi.org/10.1039/C4CC07486F
Article type
Communication
Submitted
22 Sep 2014
Accepted
05 Nov 2014
First published
06 Nov 2014

Chem. Commun., 2015,51, 232-235

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Reversible biomechano-responsive surface based on green fluorescent protein genetically modified with unnatural amino acids

J. Longo, C. Yao, C. Rios, N. T. T. Chau, F. Boulmedais, J. Hemmerlé, P. Lavalle, S. M. Schiller, P. Schaaf and L. Jierry, Chem. Commun., 2015, 51, 232 DOI: 10.1039/C4CC07486F

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