Issue 25, 2015

A simple bioluminescent method for measuring d-amino acid oxidase activity

Abstract

D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin.

Graphical abstract: A simple bioluminescent method for measuring d-amino acid oxidase activity

Supplementary files

Article information

Article type
Communication
Submitted
14 Oct 2014
Accepted
30 Oct 2014
First published
03 Nov 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 5425-5428

Author version available

A simple bioluminescent method for measuring D-amino acid oxidase activity

T. S. Bailey, M. T. Donor, S. P. Naughton and M. D. Pluth, Chem. Commun., 2015, 51, 5425 DOI: 10.1039/C4CC08145E

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