Issue 6, 2015
From the journal:

Chemical Communications

Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX†

Nasim Shahidi Hamedani,a   Fabian Blümke,a   Fabian Tolle,b   Falk Rohrbach,‡b   Heiko Rühl,a   Johannes Oldenburg,a   Günter Mayer,b   Bernd Pötzscha  and  Jens Müller*a  

* Corresponding authors

a Institute for Exp. Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
E-mail: jens.mueller@ukb.uni-bonn.de
Fax: +49 228 287 14762
Tel: +49 228 287 16735

b Life & Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany

Abstract

Short biotinylated oligodeoxynucleotides immobilized on streptavidin-coated magnetic beads allow for convenient and rapid purification of single-stranded oligodeoxynucleotides from crude asymmetric PCR mixtures, facilitating the selection of DNA aptamers.

Graphical abstract: Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX

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Article information

DOI
https://doi.org/10.1039/C4CC08233H
Article type
Communication
Submitted
17 Oct 2014
Accepted
27 Nov 2014
First published
27 Nov 2014

Chem. Commun., 2015,51, 1135-1138

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Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX

N. S. Hamedani, F. Blümke, F. Tolle, F. Rohrbach, H. Rühl, J. Oldenburg, G. Mayer, B. Pötzsch and J. Müller, Chem. Commun., 2015, 51, 1135 DOI: 10.1039/C4CC08233H

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