The 1.55 Å resolution thermostable copper nitrite reductase structure at 320 K displayed a near-bidentate binding mode of nitrite distinct from a monodentate mode in a cryogenic structure. To our knowledge, this is the first case in which the difference in substrate binding modes between cryogenic and high-temperature structures has been visualized by crystallography.
Y. Fukuda and T. Inoue, Chem. Commun., 2015, 51, 6532 DOI: 10.1039/C4CC09553G
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