Development of an acid ceramidase activity-based probe

Cécile M. J. Ouairy; Maria J. Ferraz; Rolf G. Boot; Marc P. Baggelaar; Mario van der Stelt; Monique Appelman; Gijsbert A. van der Marel; Bogdan I. Florea; Johannes M. F. G. Aerts; Herman S. Overkleeft

doi:10.1039/C5CC00356C

Development of an acid ceramidase activity-based probe†

Cécile M. J. Ouairy,^a Maria J. Ferraz,^ab Rolf G. Boot,^ab Marc P. Baggelaar,^a Mario van der Stelt,^a Monique Appelman,^b Gijsbert A. van der Marel,^a Bogdan I. Florea,^a Johannes M. F. G. Aerts*^ab and Herman S. Overkleeft*^a

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* Corresponding authors

^a Leiden Institute of Chemistry, Leiden University, P. O. Box 9502, 2300 RA Leiden, The Netherlands
E-mail: h.s.overkleeft@chem.leidenuniv.nl, j.m.f.g.aerts@lic.leidenuniv.nl
Tel: +31715274342

^b Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AS Amsterdam, The Netherlands

Abstract

Acid ceramidase is responsible for the ultimate step in the catabolism of (glyco)sphingolipids by hydrolysis of ceramide into sphingosine and free fatty acid. Deficiency in acid ceramidase is the molecular basis of Farber disease. Here we report the synthesis and characterization of an activity-based acid ceramidase probe.

Chemical Communications

Development of an acid ceramidase activity-based probe†

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