Issue 20, 2015
From the journal:

Chemical Communications

A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly†

Chenyang Tao,‡a   Yurong Yan,‡a   Hua Xiang,a   Dan Zhu,a   Wei Cheng,ab   Huangxian Juac  and  Shijia Ding*a  

* Corresponding authors

a Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P. R. China
E-mail: dingshijia@163.com
Fax: +86-023-68485786
Tel: +86-023-68485688

b The Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China

c State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China

Abstract

A novel electrochemical sensing strategy was developed for highly sensitive and specific detection of DNA by combining the exonuclease III-assisted target recycling amplification with mismatched catalytic hairpin assembly. The proposed method showed high sensitivity and selectivity with a dynamic response range from 100 fM to 5 nM.

Graphical abstract: A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC00385G
Article type
Communication
Submitted
15 Jan 2015
Accepted
03 Feb 2015
First published
11 Feb 2015

Chem. Commun., 2015,51, 4220-4222

Permissions

Request permissions

A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

C. Tao, Y. Yan, H. Xiang, D. Zhu, W. Cheng, H. Ju and S. Ding, Chem. Commun., 2015, 51, 4220 DOI: 10.1039/C5CC00385G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements