Issue 39, 2015
From the journal:

Chemical Communications

Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes†

Hiroki Ito,ab   Takuya Terai,*ab   Kenjiro Hanaoka,a   Tasuku Ueno,ab   Toru Komatsu,ac   Tetsuo Naganod  and  Yasuteru Urano*abe  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

b CREST, JST, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan

c PRESTO, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

d Open Innovation Center for Drug Discovery, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

e Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: t-terai@mol.f.u-tokyo.ac.jp, uranokun@m.u-tokyo.ac.jp

Abstract

We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities.

Graphical abstract: Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

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Article information

DOI
https://doi.org/10.1039/C5CC01613D
Article type
Communication
Submitted
23 Feb 2015
Accepted
01 Apr 2015
First published
16 Apr 2015
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2015,51, 8319-8322

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Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

H. Ito, T. Terai, K. Hanaoka, T. Ueno, T. Komatsu, T. Nagano and Y. Urano, Chem. Commun., 2015, 51, 8319 DOI: 10.1039/C5CC01613D

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