The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene

Abstract

An electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene, and it is demonstrated that the ECL biosensing method measures DNA adenine methylation methyltransferase over a dynamic concentration range (0.1 U mL⁻¹–100 U mL⁻¹) with an extremely low detection limit of 0.03 U mL⁻¹, using gold nanoparticles and a quenching ECL signal produced by a chemical quencher such as ferrocene.