Enzyme linking to DNA aptamers via a zinc finger as a bridge

Koichi Abe; Yoshiyuki Murakami; Atsuro Tatsumi; Koichi Sumida; Aki Kezuka; Takahiro Fukaya; Takenori Kumagai; Yuko Osawa; Koji Sode; Kazunori Ikebukuro

doi:10.1039/C5CC02906F

Enzyme linking to DNA aptamers via a zinc finger as a bridge†

Koichi Abe,‡^a Yoshiyuki Murakami,‡^a Atsuro Tatsumi,^a Koichi Sumida,^a Aki Kezuka,^a Takahiro Fukaya,^a Takenori Kumagai,^a Yuko Osawa,^a Koji Sode^a and Kazunori Ikebukuro*^a

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^a Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan
E-mail: ikebu@cc.tuat.ac.jp
Fax: +81 43-488-7030
Tel: +81 43-488-7030

Abstract

We propose a novel enzyme-labeling method for DNA aptamers using enzyme-fused zinc finger proteins. We achieved thrombin detection and vascular endothelial growth factor detection using zinc finger-fused firefly luciferase.

Chemical Communications

Enzyme linking to DNA aptamers via a zinc finger as a bridge†

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Enzyme linking to DNA aptamers via a zinc finger as a bridge

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