Issue 76, 2015
From the journal:

Chemical Communications

A poly(ADP-ribose) polymerase-1 activity assay based on the FRET between a cationic conjugated polymer and supercharged green fluorescent protein†

Shiyun Tang,a   Zhou Nie,*a   Wang Li,*a   Daiqi Li,a   Yan Huanga  and  Shouzhuo Yaoa  

* Corresponding authors

a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China
E-mail: niezhou.hnu@gmail.com, wli@hnu.edu.cn
Fax: +86-731-88821848
Tel: +86-731-88821626

Abstract

A label-free and convenient strategy for PARP-1 activity assay and inhibitors assessment has been developed based on the fluorescence resonance energy transfer (FRET) between a cationic conjugated polymer (CCP) and supercharged green fluorescent protein (scGFP).

Graphical abstract: A poly(ADP-ribose) polymerase-1 activity assay based on the FRET between a cationic conjugated polymer and supercharged green fluorescent protein

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Article information

DOI
https://doi.org/10.1039/C5CC04170H
Article type
Communication
Submitted
20 May 2015
Accepted
03 Aug 2015
First published
03 Aug 2015

Chem. Commun., 2015,51, 14389-14392

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A poly(ADP-ribose) polymerase-1 activity assay based on the FRET between a cationic conjugated polymer and supercharged green fluorescent protein

S. Tang, Z. Nie, W. Li, D. Li, Y. Huang and S. Yao, Chem. Commun., 2015, 51, 14389 DOI: 10.1039/C5CC04170H

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