Issue 70, 2015
From the journal:

Chemical Communications

Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging†

Wei Qiang Ong,a   Y. Rose Citron,b   Joerg Schnitzbauer,a   Daichi Kamiyamaa  and  Bo Huang*a  

* Corresponding authors

a Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA
E-mail: bo.huang@ucsf.edu

b Graduate Program of Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA

Abstract

Photoactivatable fluorescent proteins (PA-FPs) are widely used in live single-molecule super-resolution imaging but emit substantially fewer photons than organic dyes do. Herein, we show that in heavy water (D2O) instead of H2O, common PA-FPs emit 26–54% more photons, effectively improving the localization precision in super-resolution imaging.

Graphical abstract: Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging

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Article information

DOI
https://doi.org/10.1039/C5CC04575D
Article type
Communication
Submitted
03 Jun 2015
Accepted
17 Jul 2015
First published
17 Jul 2015

Chem. Commun., 2015,51, 13451-13453

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Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging

W. Q. Ong, Y. R. Citron, J. Schnitzbauer, D. Kamiyama and B. Huang, Chem. Commun., 2015, 51, 13451 DOI: 10.1039/C5CC04575D

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